Effect on bile salt

Leung

2015-05-23 3:34 am

In an investigation of the effect of bile supplementation on fat digestion in piglets,
four groups of piglets were fed with the same diet except groups of piglets were
fed with the same diet except that bile was added to fiets in the proportion of 0, 0.15%, 0.3% and 0.45% respectively for 15 days. Faecal samples were collected each day for analysis and the body weights of the piglets were monitored.

1. Suggest why it is important to monitor the body weight of the piglets in this
investigation.

2. Suggest an in vitro experimental method that allows a direct mwasurement of
the digestion of fat in a lab.

HKDSEE 2012.

Tks

https://www.sendspace.com/file/4x42p6

回答 (1)

[匿名]

2015-05-23 8:31 pm

✔ 最佳答案

1. to show that the addition of bile supplementation does not adversely affect the growth of pigs (1) / indicate the effectiveness of the bile supplementation on promoting piglets' growth/ effectiveness of fat absorption (1)

2. - prepare a mixture of lipase (bile salts) and oil (1)
- add pH indicator into the mixture / use a data logger with pH sensor / pH meter to show the change in pH of the mixture (1)
- the faster the drop in the pH of the mixture, the faster the digestion of fat (1)
- accept other reasonable experiments [oil -> filter paper -> translucent spot -> after digestion -> disappearance of translucent spot]

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