請幫我英翻中！有關HPLC測定磷脂質，急件！謝謝

[匿名]

2010-05-09 7:46 am

Determination of phospholipids by HPLC
Methanolic extracts of oils were evaporated and the residue
was dissolved in hexane/2-propanol (3:2). This solution was analyzed
by HPLC in a Waters 2695 Module (Milford, MA) equipped
with an evaporative light scattering detector (Waters 2420 ELSD).
Polar and neutral lipids were separated at 30 C using a Lichrospher
100 Diol 254-4 (5 lm) column (Merck, Darmstadt, Germany)
or a normal phase Lichrocart 250-4 (5 lm) column (Merck). In all
cases the flow rate was 1 mL min1, and samples were dissolved
in the same solvent as that used to equilibrate the column at the
time of injection. Data were processed using Empower software,
and the ELSD was regularly calibrated using commercial high-purity
standards for each lipid (Salas, Martínez-Force, & Garcés, 2006).

回答 (1)

fooks

2010-05-09 10:51 pm

✔ 最佳答案

以高效能液相色譜儀（HPLC）測定磷脂質

把油脂的甲醇萃取液蒸法後，其殘餘物溶於已烷與2-丙醇的 3:2 混合液中。以配備了蒸發光散射檢測器（Waters 2420 ELSD）的 Waters 2695 Module (Milford, MA) 高效能液相色譜儀（HPLC）把該溶液分析。使用 Lichrospher 100 Diol 254-4 (5 lm) 色譜柱 (Merck, Darmstadt, Germany) 或普相 Lichrocart 250-4 (5 lm) 色譜柱 (Merck)，把極性或中性的脂質分離。在所有檢測中，流速保持每分鐘 1 mL，而樣品均溶於注入時用以平衡譜柱的同一溶劑中。數據均以 Empower 軟件處理，而蒸發光散射檢測器（ELSD）以每種脂質商用高純度標準（Salas, Martínez-Force, & Garcés, 2006）定期校準。

（以上譯文中，儀器及化學品的廠商、型號及製造地，均保留原來英文，不另翻譯。）

參考: fooks

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請幫我英翻中 ！有關HPLC測定磷脂質，急件 ！謝謝

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請幫我英翻中！有關HPLC測定磷脂質，急件！謝謝