Although同in spite of有咩分別

Although與in spite of的意思很相似，用法上卻有分別。Although是連接詞conjunction，in spite of是介詞prepositions。所以在用法上，會因應以下英語邏輯：
Although + clause
In spite of + noun

例一：
Although it was raining heavily, we finished the basketball game.（紅字為clause）
We finished the basketball game in spite of the heavy rain.（藍字為noun）

例二：
Although he had a strong Mandarin accent, we understood what he was saying.（紅字為clause）
In spite of his strong Mandarin accent, we understood what he was saying.（藍字為noun）

In spite of, despite and although are all used to show a contrast but there are differences in the structures used with them.

003 is more close to a perfect answer. The concept of 004 is wrong. Don't follow her!

Although -　雖然...

Although it rains/ed, we do/did not cancel the hiking trip.
雖然下雨，我們沒有取消遠足行程。
雖然有一種情況，理論上應該是某結果，但是表達另一樣結果。

In spite of - 即使/縱使

In spite of the rain, we still go/went hiking.
縱使下雨，我們仍去遠足(行山)。
不論前因如何，也不會改變計劃/決心。

特登用兩句意思相近的作例子：
微妙之處在於in spite of是表達不論處境如何,後面表達不會因為前述的情況而改變要達成的結果；但although或but意義上是表達前後兩件狀況的對比應該出現的結果的相反之處。

I think you know the difference of the meaning from it. And the biggest difference is Although follows with sentence, in spite of + noun.
E.g. Although she was hungry, she didn't buy anything from the supermarket
E.g. He continued to singin spite of people's objection

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