✔ 最佳答案
If you don't have access to disposible loops, you can make one from wire, use the tip of a sharp pencil to form the loop, that will be about the size of a 10 uL. You will need to flame the loop, give it a minute to cool then transfer the colony to the desired plate. Use either a 3 or 4 way streak for Isolation.
You can always use it like a needle too, just be careful not to plough up the agar.
Used to have to do it that way til we got sterile loops.