Enzyme activity 與 benedict solution 有關係嗎?[20分]

The copper(2) sulphate in Benedict solution is simply for formation of a red precipitate to indicate the presence of the monosaccharides (simple sugars such as glucose). Benedict shouldn't inhibit enzyme activity because it is commonly used in enzyme tests/labs for the presence of simple sugars.

Your result maybe due to 2 reasons:

1) the enzyme used didn't have enough time to break apart sucrose into glucose and fructose molecules, thus, benedict solution couldn't detect the presence of simple sugars. Since benedict cannot detect sucrose, it didn't form any precipitate. Once everything started boiling, the enzyme is inactivated because it denatures under high temperature, therefore, there won't be any glucose/fructose molecules to detect.

2) the enzyme you used maybe ineffective in breaking sucrose to glucose and fructose, thus, benedict can't detect any simple sugars

我係1個中5 student.

sucrose 唔係reducing sugar
所以只+ Benedict's solution 會出negative result

method: 先+ HCl 再+benedict's solution boil shows +ve result