Extraction and Isolation. The dried leaves (1.2 kg) were extracted
three times with MeOH overnight at room temperature to give 160 g
of crude extract. The extract was partitioned between EtOAc and H2O
to produce an EtOAc-soluble fraction (fraction A, 29.5 g). Using
n-hexane and MeOH-H2O (4:1), fraction A was divided into an
n-hexane and a MeOH-H2O layer. The MeOH-H2O layer exhibited
cytotoxicity against the HepG2 (IC50 ) 11.0 íg/mL) and A549 (IC50
) 19.5 íg/mL) cell lines.