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2007-12-31 8:28 am
Extraction and Isolation. The dried leaves (1.2 kg) were extracted
three times with MeOH overnight at room temperature to give 160 g
of crude extract. The extract was partitioned between EtOAc and H2O
to produce an EtOAc-soluble fraction (fraction A, 29.5 g). Using
n-hexane and MeOH-H2O (4:1), fraction A was divided into an
n-hexane and a MeOH-H2O layer. The MeOH-H2O layer exhibited
cytotoxicity against the HepG2 (IC50 ) 11.0 íg/mL) and A549 (IC50
) 19.5 íg/mL) cell lines.

回答 (2)

2007-12-31 10:28 am
✔ 最佳答案
萃取與分離 乾燥葉(1.2 kg)用甲醇室溫下過夜萃取 連續三次得到160g粗抽出物
粗抽出物以乙酸乙酯:水 分亦漏斗萃取 乙酸乙酯層可得 分段組成A, 29.5 g
再以正己烷 和 甲醇-水 (4:1)進行萃取
分段組成 A可得到正己烷 和 甲醇-水兩層
甲醇-水層顯示對於HepG2 (IC50 ) 11.0 íg/mL) and A549 (IC50
) 19.5 íg/mL)具有細胞毒性
2007-12-31 10:08 pm
提取和隔離。乾葉子(1.2 公斤) 隔夜被提取了三次與MeOH 在室溫給160 g 粗暴萃取物。萃取物被分成在EtOAc 和H2.O 之間生產一個EtOAc 可溶解分數(分成幾部分A, 29.5 g) 。使用正己烷和MeOH-H2.O (4:1), 分成幾部分A 被劃分了成正己烷和MeOH-H2.O 層數。MeOH-H2.O 層數被陳列的cytotoxicity 反對HepG2 (IC50) 11.0 3ig/mL) 和A549 (IC50) 19.5 3ig/mL) 細胞排行。


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