Assigned buffer : Tris-HCl (pH=9,0.05M,250mL )
*Tris M.W.=121.1,
Pka of acid component= 8.30,
conc. of HCl=0.1M
1 : 1 : 1
Tris base + HCl ---> Tris acid
Calculate the correct amount of the reagents requied to prepare 250mL of assigned buffer.