preparation of Buffer 15分

2006-10-21 6:02 pm
Assigned buffer : Tris-HCl (pH=9,0.05M,250mL )

*Tris M.W.=121.1,
Pka of acid component= 8.30,
conc. of HCl=0.1M

1 : 1 : 1
Tris base + HCl ---> Tris acid

Calculate the correct amount of the reagents requied to prepare 250mL of assigned buffer.

回答 (1)

2006-10-21 10:12 pm
✔ 最佳答案
I am a chemistry teacher for more than 20 years. I don't think any body can help you.
What is Tris? What is the Ka or Kb of the said compound? How to help?


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